2 edition of novel dielectric technique for monitoring the lyophilisation of globular proteins found in the catalog.
novel dielectric technique for monitoring the lyophilisation of globular proteins
Phe Man Suherman
Thesis (Ph.D) - De Montfort University, Leicester 2001.
|Statement||by Phe Man Suherman.|
|Contributions||De Montfort University.|
Advanced models including anisotropic curvature and molecular details. Globally, mechanics-based methods for in vitro assays in their current form capture the essentials of membrane shaping by BAR domains, but fail to distinguish the structural details. So far, although some theoretical models include an anisotropic spontaneous curvature for the BAR domains, only an isotropic spontaneous Since protein dynamics is related to protein function and essential transport processes, a detailed mechanistic understanding and monitoring of protein dynamics in solution is highly desirable. The hierarchical character of protein dynamics requires experimental tools addressing a broad range of time- and length ://
Dye ligand chromatography: A unique and selective purification technique in which ligands may act as substrate analogs, offering affinity interactions with their corresponding enzymes, e.g. the interaction of Cibacron blue F3GA with proteins and enzymes. Another example of synthetic affinity ligands is chlorotriazine dyes, often used as The book then explores a variety of practical topics, including: Principles and applications of important polymer characterization tools, such as light scattering, gel permeation chromatography, calorimetry, rheology, and spectroscopy Automatic continuous online monitoring of polymerization (ACOMP) reactions, a flexible platform that enables
Proc. SPIE , Time-Resolved Laser Spectroscopy in Biochemistry III, pg 20 (1 April ); doi: / Proteins interact ︁ in a transient yet specific manner to relay signals within the crowded cellular environment. In their Communication on page ff., C. Tang, W.‐P. Zhang, and co‐workers show that phosphorylation signaling can take place between two proteins with a binding affinity of only 25 m M and a complex lifetime in the μs–ms range, which is an order of magnitude weaker
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A NOVEL DIELECTRIC TECHNIQUE FOR MONITORING THE LYOPHILISATION OF GLOBULAR PROTEINS. View/ Open. Suherman, Phe () PhD (Mb) Date A novel dielectric technique for monitoring the lyophilisation of globular proteins.
Thesis (Thesis) Find all citations by this author (default) A NOVEL DIELECTRIC TECHNIQUE FOR MONITORING THE LYOPHILISATION OF GLOBULAR PROTEINS Suherman, Phe Man (Thesis or dissertation / Doctoral / PhD) Background.
The moisture content of lyophilised proteins plays an important role in the stability and long-term storage of these substances. Residual moisture is mainly determined by the combination A NOVEL DIELECTRIC TECHNIQUE FOR MONITORING THE LYOPHILISATION OF GLOBULAR PROTEINS Suherman, Phe Man (Thesis or dissertation / Doctoral / PhD) Background.
The moisture content of lyophilised proteins plays an important role in the stability and long-term storage of these substances. E-infrastructures are emerging as novel and effective The amount of non-frozen water for globular proteins is about – g g −1 protein, slightly less than the proteins’ hydration shell (Rupley and Careri,Kuhlman et al., ).
More detailed analysis of each lyophilization step is provided in Section :// Polarized light microscopy is a simple, quick, and sensitive technique to detect solid states including crystal forms and hydrates, and the overall crystallinity of API and/or excipients as well as the morphology of freeze-dried pharmaceutical proteins.
In general, the presence of birefringence patterns confirms the presence of crystals in the N 4-Macrocyclic Metal Complexes Edited by José H. Zagal (Universidad de Santiago de Chile), Fethi Bedioui (Ecole Nationale Supérieure de Chimie de Paris, France), and Jean-Pol Dodelet (INRS-Énergie, Matériaux et Télécommunications, Varennes, Québec, Canada).Springer Science + Business Media, LLC: New York.
xvii + pp. $ ISBN Book Review Book Review BOOK REVIEWS concepts. A more than sufficient number of references () are listed. Although following the text around inserts and tables was difficult in some places, the text is well written and easily understood with the presentation of numerous figures to clarify the :// Smith, G, and Polygalov E.
Apparatus for measuring the dielectric properties of conductive materials. British patent Filing date 13 March Publication number GB 17 September Smith, G., Polygalov, E.
and Page, T. () A method for monitoring and/or controlling process parameters of a lyophilisation :// book reviews Macromolecules Containing Metal and Metal-Like Elements, Volume 3: Biomedical Applications Edited by Alaa S.
Abd-El-Aziz (The University of Winnipeg), Charles E. Carraher, Jr. (Florida State University), Charles U. Pittman, Jr. (Mississippi State University), John E. Sheats (Rider University), and Martel Zelden (Hobart and William A novel technique such as dielectrophoresis (DEP) can be used as an alternative to the traditional protein purification and separation methods for proteins, since DEP can achieve manipulation of biomolecules in gel-free environment as well as rapid separation and preconcentration capability .
This work therefore investigates novel low-cost skin aging assessment technique and equipment by using robust analysis of textural features unified with a laser-speckle imaging method, which is Phe Man Suherman Acknowledgement December De Montfort University PhD.
Thesis i Acknowledgement I would like to express my gratitude to a number of people for their contributi A lyophilization process is usually only specified in terms of a ‘recipe’ (shelf temperature and chamber pressure vs.
time); according to the recent PAT guidelines issued by FDA, there is the Ion Source. A suitable ESI source for the mass-spectrometric analysis was designed by the Fenn group in the mid s [4, 18–20].Later on it was modified by different research groups to improve the system's robustness [21–25].Generally a dilute (less than mM in polar volatile solvent) analyte solution is injected by a mechanical syringe pump through a hypodermic needle or stainless The aim of this work was to develop a minimally-invasive, impedance spectroscopy method as a novel process analytical technology for monitoring the freeze drying :// Therapeutic proteins are the proteins which are designed for the individuals in whom proteins are absent or low as in the case of cancer, hemophilia, anemia, multiple sclerosis, hepatitis B/C, etc.
The idea of therapeutic proteins was first conceptualized in the s by engineering insulin for the treatment of The first section of the book deals with chemical and mechanical processing and engineering of biomaterials, tailored towards specific biomedical purposes.
The second section presents 6 chapters reporting novel applications of biomaterials to nanomedicine and drug delivery. Finally, 9 chapters have been gathered to show Nanotechnology and Nanomaterials. Nanotechnology is the art and science of handling matter at the nanoscale (down to 1/10, the width of a human hair) to create new and unique materials and products with properties that differ significantly from those on a larger developments in nanotechnology were initiated by Nobel Laureate Richard Feynman, who introduced In this paper, we elucidate the information gained in dielectric spectroscopy (DS) and electrochemical impedance spectroscopy (EIS) and how these techniques may be of importance for future protein DEP manipulation.
EIS and DS can be used to determine the dielectric properties of proteins predicting their DEP ://. This study investigates the temperature dependence of the low frequency dielectric properties ( Hz–1 MHz) of hydrated globular proteins (namely, ovalbumin, lysozyme and pepsin).Globular proteins with acidic isoelectric points, such as Downloaded by [Tufts University] at 27 November BSA, are precipitated more readily by tannin at pH than at pHand basic proteins such as lysozyme have higher affinities for polyphenol binding at higher pH (Hagerman and Butler, ).Electrochemical immunosensors, EIs, are systems that combine the analytical power of electrochemical techniques and the high selectivity and specificity of antibodies in a solid phase immunoassay for target analyte.
In EIs, the most used transducer platforms are screen printed electrodes, SPEs. Some characteristics of EIs are their low cost, portability for point of care testing (POCT